For studies of factors which have influence to Donaggio-titer of α1-acid glycoprotein and albumin in urine, the effect of running in summer was researched and following results were obtained. 1) Increase in both actual and corrected to a urine density of 1,024 Donaggio titer was observed in the urine after a marathon running. (P=0.01 throughout all days.) 2) Increase in both actual and corrected urinary α1-acid glycoprotein was observed with students in the urine after a marathon running. 3) Increase in urinary albumin was observed second and third day of 5 days after a marathon running. 4) Discussion was made on High Level of urinary Donaggio-titer and glycoprotein and albumin. after running in the first day.