The effects of six fixatives (Carnoy, Allen, Gendre, Rossman, Glutaraldehyde and Periodic acid-formalin) on the distribution of glycogen of the liver were studied by light and electron microscope. In tissue fixed with Carnoy, Allen, Gendre and Rossman, liver cells showed an alcohol flight phenomenon and chess board pattern was observed in the center of the tissue. The results obtained from freeze-drying method, that is, the alcohol flight phenomenon was an artifact, but the chess board pattern was not. In tissue fixed with glutaraldehyde and periodic acid-formalin, the alcohol flight phenomenon was rarely observed but the chess board pattern was clearly recognized. To study the effect of the temperature of fixatives on the changes in glycogen distribution, liver was fixed with glutaraldehyde and periodic acid-formalin at various temperatures. Good results were obtained with periodic acid-formalin under 20℃ or with glutaraldehyde under 4℃. Electron microscopy showed that five fixatives (except glutaraldehyde) caused cell disruption and fine ultrastructure was not observed. Glycogen was observed as β and α particles with glutaraldehyde fixation. The effect of pH of glutaraldehyde solution on glycogen distribution was not discernible. These results showed that fixation with glutaraldehyde was the best method to study glycogen particles by both light and electron microscope.