In an attempt to know the distribution of Clonorchis sinensis, investigations have been conducted in Chugoku District except for Okayama Prefecture. 1. Parafossalurus manchouricus, the first intermediate host of the fluke, were found in the southern part of Fukuyama City, Hiroshima Prefecture and also in Natajima, Yamaguchi City, Yamaguchi Prefecture. 2. Metacercariae of Clonorchis sinensis were recognized in 6 of 56 (61.5%) Pseudorasbora parva collected and the incidence was significantly high in the southern section of the area. 3. Centrifugal fecal examinations were performed at Natajima and eggs of Clonorchis sinensis were recovered from 12 of 218 (5.5%) residents. Among cats dissected, 4 of 12 (30.0%) were found to be infected with liver flukes.