Stdudies on surface structures of the bacterial cell. Report 11. Physico-chemical studies on surface structures of bacterial cell utilizing dielectric spectrum

In the dielectric spectra of bacterial cell, there found three kinds of abnormal distribution and absorption of electric wave which widely distributed from ultra-short wave to centimeter wave. These absorption curves were named as first, second and third curve and could be completely analyzed by Cole-Cole procedure. Thus detailed analyzed data indicated that these curves were preusmably and theoretically due to three different kinds of binding water. To determine the intracellular locations of these binding water, effect of pH on their spectra and isothermic changes of absorption curve were investigated. Fffect of pH revealed that they were completely different from each other demonstrating double dissociation curve in the first, simple dissociation curve in the second, and no special dissociation curve in the third. Isothermic changes of spectra were also analyzed by Freundlich and Cunningham's equation. It was concluded from this analysis that there were at least two kinds of absorption centre in the first, one kind in the third. However the second could not be explained by multi-molecular absorption. On the other hand, changes of spectra in agglutination reaction, difference between S-type and R-type of bacterial cell, changes during growth of bacterial cell, effects during the process of production of adaptation-enzyme were also investigatcd. Thus, it was concluded that first absorption was originated from polysaccharide in surface substance and second absorption was due to intracellular unknown substance. On the other hand, third absorption was considered to be due to polypeptide in surface substance.