Journal of Okayama Medical Association
Published by Okayama Medical Association

Full-text articles are available 3 years after publication.

呼吸酵素系の組織化学的並に生化学的研究 第2編 組織内チトクローム酸化酵素活性の比色測定法について

Okazaki, Hiroaki
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Basing on the theory presented in the previous report, the author described in the present report the new colorimetric methods for the estimation of the activities of cytochrome c oxidase and of cytochrome c-cytochrome oxidase system in tissue homogenates using neotetrazolium chloride in the presense of p-phenylenediamine with or without additional cytochrome c. Moreover, colorimetric estimation of cytochrome c-cytochrome oxidase system activity in various tissues such as the kidney, heart, liver, brains, and skeletal muscle was made using this methods.