Journal of Okayama Medical Association
Published by Okayama Medical Association

Full-text articles are available 3 years after publication.

P(32)による実験的流行性肝炎の研究 第2編 岡山県下に発生せる所謂流行性肝炎病毒接種マウス肝内に於ける燐代謝のSchmidt-Thanhauser法による研究

Tanabe, Kenya
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In the past few years, the pathogene of infectious hepatitis occured in Okayama prefecture was isolated, and the study of characteristics of its pathogene were carried out in our department. Previously the author observed the significant changes on nucleic acid fraction by the investigation of phosphorus metabolism of the pathogene-inoculated mouse liver and reported its results in part Ⅰ. The author studied further the phosphorous metabolism of DNA fraction and RNA-protein fraction after the fractionation of phophorous compound by the Schmidt-Thanhauser's method and obtained the following results, 1) The P(32) incorpoation into DNA fraction was increased at the 6th and 7th day of the inoculation, the maximum incorporation was found at the 12th and 13th day. However, the incorporations at the 22nd and 23rd day showed no difference against control group. Hence, the intense change of phosphorus metabolism was presumed to occur at the early stadium after the inoculation. 2) The P(32) incorporation into RNA-protein fraction was not increased at the early stadium, but the incorporation showed the maximum at the 12th and 13h day; the maximum incorporation indicated the occurence of increased specific or non-specific metabolism in the cell. At the late stadium, the difference in the incorporation between tested and control group was not observed. 3) In the dilution test of the pathogene, it was found the serious affection on phosphorus metabolism of mouse in the dilution of 10(-4) or 10(-6). 4) These results showed the similar tendencies in the phosphorus metabolism as part Ⅰ in which the study was carried out by means of Schneider's method. The occurence of increased phosphorus metabolism of DNA fraction at the early stage after inoculation presumablly indicated that the pathogene investigated contained DNA as main nucleic acid just alike animal viruses.