Journal of Okayama Medical Association
Published by Okayama Medical Association

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Full-text articles are available 3 years after publication.

精製細菌鞭毛に依る凝集反応の電子顕微鏡的観察法に就て

Shida, Tsuguo
Fukushima, Satoshi
Ishii, Seiichi
Nakashima, Tadaatsu
Nikuma, Kohichiro
Suga, Masao
Thumnail 71_163.pdf 1.01 MB
Abstract
The flagella of Bacillus Proteus were purified according to Fukumi's method. To these purified flagella, antiserum and human γ-globulin were added, and the morphological change was electron microscopically observed. The results were briefly summarized as follows: 1) For the purification of flagella, homogenation for 15 seconds is the best. 2) The purified flagella of Bacillus proteus had a rope structure. 3) After the addition of antiserum, agglutination figure of flagella was electron-microscopically observed. 4) The enlargement of flagella, however, could not be observed after the addition of antiserum. 5) The grade of agglutination of flagella goes in parallel with the grade of dilution of antiserum 6) The addition of human γ-globulin did not cause agglutination, and there was observed only the adsorbing figure of globulin particles on them.
ISSN
0030-1558
NCID
AN00032489