In part I, the multiplication of the infectious hepatitis virus in the chickembryo was discussed. In the present part, the relation between the multiplication of the virus and infectious routes was studied. The results are briefly summarized as follows: 1) Infection of the hepatitis virus in the chickembryo had been determined by life or death and pathologic changes in the chickembryo. The author found, however, that the infection of the virus was better determined by inoculation of various organs of the infected chickembryo into mice and by observation of pathologic changes in thus inoculated mice. 2) Of all the infectious routes in the chickembryo, allantoic inoculation gave the most excellent result showing the virus distribution over all parts of the embryonated egg. The other routes gave no accurate result. 3) The infection was established more easily by a highly diluted virus emulsion (×10⁹) than by a thick one (×10²). As the reason for this phenomenon, two ones are considered, the inhibition of the virus aefivity by intracellular non-specific inhibitive substances and the reciprocal interference among the viruses. It could not be clarified, however. which of the two was the real one.