In observation on the carbohydrate metabolism of bone marrow of normal rabbits by bonemarrow tissue culture (Carrel's flask method) and on the effect of normal human serum on carbohydrate metabolism in the same culture, the following results have been obtained: 1) Sugar consumption increases in proportion to the increase in the rate of tissue frowth in culture. 2) Hydrogen ion concentration in substrate tends to turn acidic along with progress of the tissue growth. 3) With an addition of a small quantity of normal human serum the tissue growth is markedly accelerated, and both sugar consumption and the increase in hydrogen-ion concentration are also promoted. 4) With a large amount of normal human serum added to culture medium, on the other hand, the tissue growth is inhibited and gugar consumption and the increase in the hydrogenion concentration are less. 5) When a proper amount of normal human serum is added, no effect is observable on the tissue growth, sugar consumption and on hydrogen-ion concentration.