In the present report, the author investigated the complement-fixing antigenicity of the albumin, globulin, polysaccharide and tissue fractions of the peritoneal exudate of guinea-pigs infected with Rickettsia tsutsugamushi. Mitani and Tanizawa strains of R. tsutsugamshi were used as the test agents. Each fraction was obtained, in such a way as mentioned in report Ⅰ, from the peritoneal exudate of guinea-pigs previously injected with chloipromazine and then inoculated with rickettsiae. The antisera were obtained from the rabbits immunized by inoculation of rickettsiae into the vein. Against each of the antisera of Mitani and Tanizawa strains, both of albumin and tissue fractions showed a high antigenicity, while globulin and polysaccharide fractions showed no noticeable antigenicity. From this result, it is concluded that albumin and tissue fractions play a chief role in the complement-fixing antigenicity of the peritoneal exudate of rickettsia-infected guinea-pigs.