1) The variation of measurement of the brain water content caused by the different concentration of the cobaltchloride solution was estimated. The solutions of 9.58g/dl, 9.8g/dl, 10.0g/dl, 10.3g/dl and 10.6g/dl were used upon the same material. 2) The free wafer content increased according to the dilution of the solution in normal cases. 3) A similar result to the normal one was seen in cases of the brain edema, but more distinctly. 4) The cobaltchloride method was compared with the drying method in normal cases. 10.0g/dl solution showed the most similar result to the drying method. 5) The water content became more in the case using weaker solution than 10.0g/dl. Using a higher concentration, the brain would be dehydrated and the brain swelling might he overlooked. 6) From the above-mentioned the 10.0g/dl solution is the best to be used.