The glycogen content in both livers and muscles as well as the blood sugar values were measured after letting guinea pigs standing for 2 hours at room temperature or at 41°. After imposition of a definite dose of India ink, lithion-carmine and “Koha” of neo-cyamine and in some cases together with vitamin C, and the following results were obtained: 1. By imposing these drugs, liver glycogen decreased slightly in India ink and pretty in lithion-carmine cases. 2. Liver glycogen content much more decreased by exposing the animal to high temperature after imposing these drugs, and the degree between India ink and lithion-carmine cases was almost the same, but, from the viewpoint of the degree of the decrease caused by drug itself, the India ink case was strongly influenced by high temperature. In a similar condition. liver glycogen content of the “Koha”-imposed was markedly high than the nonimposed. 3. The decrease of liver glycogen content under high temperature after imposition of these drugs. was slightly inhibited in India ink case by simultaneous imposition of vitamin C, while accelerated contrarily in the other cases by it. 4. Muscle glycogen content showed almost no change in India ink case, and it increased contrarily in lithion-carmine case. 5. Muscle glycogen content always decreased by exposure to high temperature after imposing these drugs, and vitamin C showed scarcely any effective action on these conditions. 6. The blood sugar values stayed at normal range in India ink case, but decreased in lithion-carmine case. It, furthermore, always increased by further imposition of vitamin C under high temperature. These influences to increase or decrease the value were most apparent in lithion-carmine case.