The measurements of the fatigue on conductors, porters, locomotive-drivers and locomotive-firemen, were studied by Donaggio-Sato-reaction and serological test. The results were as follows: 1) Trainmen worked for a long time on the train had an accumulation of fatigue on the next day. 2) The porter's fatigue proved greater than conductor's by upper methods. 3) The quantity of urine-protein in trainmen and locomotive-operators was greater than that of workers of lighter kind. 4) The quantity of urine-protein differed individually. 5) The serological test of urine protein, Donaggio reaction and congored-test seemed to have some interrelation among them.