The acute pancreatic necrosis of dogs was performed experimentally by injecting olive oil or bile (of the same dog or oxen) into the pancreas through the pancreatic duct. The experimental cases were classified into three groups i.e. (I) SERIOUS, (II) MEDIUM, and (III) MILD. On the cases of the 1st group, bleeding and fatty necrosis were remarkably observed and the dogs usually died within 72 hours after the operation. The dogs of the 3rd group were alive, but only atrophy or edema of pancreas were recognized. The cases of the 2nd group were those whose conditions are considered between those two groups. The examination was performed in the following methods. Specific gravity of whole blood and plasma. by Copper sulfate method. Hematocrite: Anticoagulated blood was sucked into a Wintrob's tube, and was centrifuged by the centrifuge at 3,000 turning per minute for 30 minutes. Total serum protein. By protein refractometer (Hitachi). Blood volumes and serum volumes: By Gregerson's method by using Evans blue, serum was taken in 10 minutes after Evans blue was injected. The following results were obtained On the cases of the serious group, specific gravity of blood and hematocrite's value increased markedly and specific gravity of plasma and total serum protein increased temporarily. Blood volume and serum volume decreased markedly. The dogs of this group were suffered from shocks which caused them died. On those of the medium and mild groups, at the beginning stage of the disease, blood thickening was observed, but after 48 hours passed, there occurred a marked decrease of specific gravity of blood and plasma, hematocrite and total serum protein. And it was not improved easily.