Journal of Okayama Medical Association
Published by Okayama Medical Association

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浮遊培養によるニワトリ胚網膜色素上皮細胞からの水晶体様構造の形成

Tsutsui, Yasuko
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Abstract
We observed the morphologic changes of tissue fragments of retinal pigment epithelium (RPE) of chick embryos in floating culture under the presence of bacic fibroblast growth factor (bFGF). At higher concentration (10 and 200 ng/ml) of bFGF, rolled up RPE sheets grew to develop non-pigmented lumps with the structure like onions, while dissociated RPE cells did not from such structure. Histologically, the onion-like structure consisted of lens-fiber-like cells, positive for anti-crystallin antibody and anti-bFGF-receptor antibody. This lentoid structure was not formed at 1 ng/ml or lower concentration of bFGF. The trans differentiation from RPE cells to lens epithelial cells was dependent upon the concentration of bFGF as well as the proper interaction among RPE cells.
Keywords
浮遊培養
網膜色素上皮細胞
分化転換
水晶体様構造
bFGF
Note
原著
ISSN
0030-1558
NCID
AN00032489