Journal of Okayama Medical Association
Published by Okayama Medical Association

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尿中 NRK49-F 細胞増殖促進活性の各種腎疾患の病態における役割

Eguchi, Junji
106_875.pdf 1.78 MB
Abstract
To evaluate the roles of transforming growth factor-β (TGF-β) on the glomerular cell proliferation and sclerosis, a new colorimetric bioassay system for urinary excretion of TGF-β has been developed by using NRK49-F cell culture. The urinary TGF-β activity was detected by this method in focal glomerular sclerosis (FGS) and purpura nephritis but not in minimal change nephrotic syndrome and IgA nephropathy. The bioassayed TGF-β activity was comfirmed by a blocking experiment using a neutralizing antibody and by Western immuno-blotting. These findings suggest that TGF-β plays an important role in the progression of the glomerular leisions, in FGS and purpura nephritis.
Keywords
TGF-β
NRK49-F
colorimetric bioassay
Note
原著
ISSN
0030-1558
NCID
AN00032489