Journal of Okayama Medical Association
Published by Okayama Medical Association

<Availability>
Full-text articles are available 3 years after publication.

アスコルビル酸による 1,25dihydroxyvitaminD(3) 作用の増強―培養ヒト骨肉腫細胞 MG63 における検討―

Kurihara, Makoto
Thumnail 104_617.pdf 1010 KB
Abstract
We eximined the interaction between 1,25-dihydroxyvitamin D(3) and ascorbic acid in the human osteosarcoma cell MG63 by measuring alkaline phosphatase (ALP) activity. Both vitamins induced ALP activity in this cell line. Simultaneous addition of both ascorbic and 1,25-dihydroxyvitamin D(3) induced much higher ALP activity than each vitamin alone. Pretreatment of MG63 with ascorbic acid or type I collagen, increased the ALP activity induced by 1,25-dihydroxyvitamin D(3). Binding assay anf immunoblotting analysis showed that the number of vitamin D receptors was increased in MG63 pretreated with ascorbic acid. In conclusion,ascorbic acid stimulates collagen production of MG63, and collagen increases the vitamin D receptor content. The increase in vitamin D receptor content potentiates the activity of 1,25-dihydroxyvitamin D(3) in this cell line.
Keywords
MG63
1,25dihydroxyvitaminD(3)
ascorbic acid
type I coollagen
vitaino D recepter
ISSN
0030-1558
NCID
AN00032489