Journal of Okayama Medical Association
Published by Okayama Medical Association

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Oncogene mRNA in gastric cancer by in situ hybridization by using a biotin-labeled probe

Tanaka, Izuru
103_247.pdf 5.98 MB
Published Date
1991
Abstract
Expression of oncogene mRNA in gastric cancer was studied by in situ hybridization. Cancer tissues were fixed in formalin and embeded in paraffin. Then, DNA-mRNA hybridization was performed by using two biotin-labeled cDNA probes (c-myc, c-Ha ras). Oncogene mRNA was intracuelularly localized, mainly in the cytoplasm, and also found in the nucleus of partial cells. Higher concentrations of oncogene mRNA vere found in advanced cancers than in early cancers, and in metastatic positive cases of the above n2 than of n0 or n1. This suggests that the expression of oncogene mRNA is related to the malignant potency of cancer.
Keywords
histo in situ hybridization 法
胃癌
癌遺伝子
mRNA
Note
原著
ISSN
0030-1558
NCID
AN00032489