Journal of Okayama Medical Association
Published by Okayama Medical Association

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Full-text articles are available 3 years after publication.

肝細胞 spheroid に観察される vermipodia 様細胞突起

Tanabe, Takayoshi
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Abstract
The presence and function of vermipodia-like processes of hepatocytes-multicellular spheroids formed in primary culture was described. Cine microscopy and scanning electron microscopy revealed that about 1-5% hepatocytes in single cell after culture initiation already posessed the processes. The processes disappeared when the hepatocytes attached and spread, and than appeared again on the surface of multicellular spheroides. The processes were moving by extending, shrinking and twisting, and sometimes extended 50 um. Five to ten percent of spheroids had 1-5 processes. Occasional collision of two spheroids resulted in the fusion of spheroids after their processes intertwined, attached to the partner spheroid and shrunk. Electron microscopy revealed that the processes were larger in size than the numerous microvilli co-present on the free surface of spheroids and their cytoplasm had scarece orgalella. The morphological characterisitcs of the processes most resembled those of vermipodia reported in histiocytic leukemia cells.
Keywords
ラット肝細胞
初代培養
細胞突起
細胞癒合
ISSN
0030-1558
NCID
AN00032489