Bulletin of Faculty of Health Sciences Okayama University Medical School
Published by Faculty of Health Sciences Okayama University Medical School

<Formerly known as>
岡山大学医療技術短期大学部紀要 (1巻-9巻)

ヒト血清M蛋白のIgGH鎖サブクラスの型判定について -高分解能アガロースゲル等電点電気泳動法の分離と検出のための条件設定-

Ichimura, Mitsuko
Tohge, Hiroko
Sakiyama, Junko
Nakata, Yasunari
Endo, Hiroshi
Abstract
IgG Heavy-Chain subclass typing of human serum M-protein were isoelectrofocussed (IEF) in agarose gel, and then the bands of IgG were detected with peroxidase-conjugated anti-human IgG1-4 monoclonal antibodies on the same isoelectrofocussed agarose gel plate. This IEF enzyme immunodetection patterns were composed of four to eight discrete bands (The range of pI was 6.0 to 9.0). These bands were dependent on immunofixationbands. It was very specific and clear to detect the subclass of IgG Type M-protein in human serum using this method.
Keywords
IgG Subclass
M-Protein
Isoelectrofocusing
Monoclonal Antibodies
ISSN
0917-4494
NCID
AN10355371