Acta Medica Okayama volume74 issue4
2020-08 発行

D-Tagatose Effectively Reduces the Number of Streptococcus mutans and Oral Bacteria in Healthy Adult Subjects: A Chewing Gum Pilot Study and Randomized Clinical Trial

Nagamine, Yuichi Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Kagawa University
Hasibul, Khaleque Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Kagawa University
Ogawa, Takaaki Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Kagawa University
Tada, Ayano Department of Microbiology, Faculty of Medicine, Kagawa University
Kamitori, Kazuyo Department of Molecular Physiology and Biophysics, Faculty of Medicine, Kagawa University
Hossain, Akram Department of Molecular Physiology and Biophysics, Faculty of Medicine, Kagawa University
Yamaguchi, Fuminori dDepartment of Medical Technology, Division of Fundamental Medical Technology, Ehime Prefectural University of Health Sciences
Tokuda, Masaaki eInternational Office, Kagawa University
Kuwahara, Tomomi Department of Microbiology, Faculty of Medicine, Kagawa University
Miyake, Minoru Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Kagawa University
Publication Date
2020-08
Abstract
We examined the effect of D-Tagatose on the growth of oral bacteria including Streptococcus mutans (S. mutans). Saliva collected from 10 healthy volunteers was plated on BHI medium (to culture total oral bacteria) and MBS medium (to culture S. mutans, specifically). Agar plates of BHI or MBS containing xylitol or D-Tagatose were cultured under aerobic or anaerobic conditions. We then counted the number of colonies. In BHI plates containing D-Tagatose, a complete and significant reduction of bacteria occurred under both aerobic and anaerobic conditions. In MSB medium, significant reduction of S. mutans was also observed. We then performed a doubleblind parallel randomized trial with 19 healthy volunteers. They chewed gum containing xylitol, D-Tagatose, or both for 4 weeks, and their saliva was collected weekly and plated on BHI and MSB media. These plates were cultured under anaerobic conditions. Total bacteria and S. mutans were not effectively reduced in either the D-Tagatose or xylitol gum group. However, S. mutans was significantly reduced in volunteers chewing gum containing both D-Tagatose and xylitol. Thus, D-Tagatose inhibited the growth of S. mutans and many types of oral bacteria, indicating that D-Tagatose intake may help prevent dental caries, periodontitis, and many oral diseases.
Document Type
Original Article
Keywords
D-Tagatose
xylitol
Streptococcus mutans
oral bacteria
chewing gum
Link to PubMed
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