ID 58730
Author
Okazaki, Yuki Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Furumatsu, Takayuki Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Kaken ID publons
Kamatsuki, Yusuke Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Nishida, Keiichiro Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Kaken ID publons researchmap
Nasu, Yoshihisa Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Nakahara, Ryuichi Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Saito, Taichi Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences ORCID Kaken ID
Ozaki, Toshifumi Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Kaken ID publons researchmap
Abstract
Background
Many histological, mechanical, and clinical studies have been performed on the medial meniscus posterior root attachment, as it often tears in patients with osteoarthritic knee. Medial meniscal root repair is recommended in clinical situations; however, to date, no studies have examined the differences between meniscal root and horn cells. The aim of this study was, therefore, to investigate the morphology, reaction to cyclic tensile strain, and gene expression levels of medial meniscal root and horn cells.
Methods
Meniscal samples were obtained from the medial knee compartments of 10 patients with osteoarthritis who underwent total knee arthroplasty. Root and horn cells were cultured in Dulbecco's modified Eagle's medium without enzymes. The morphology, distribution, and proliferation of medial meniscal root and horn cells, as well as the gene and protein expression levels of Sry-type HMG box 9 and type II collagen, were determined after cyclic tensile strain treatment.
Results
Horn cells had a triangular morphology, whereas root cells were fibroblast-like. The number of horn cells positive for Sry-type HMG box 9 and type II collagen was considerably higher than that of root cells. Although root and horn cells showed similar levels of proliferation after 48, 72, or 96 h of culture, more horn cells than root cells were lost following a 2-h treatment with 5% and 10% cyclic tensile. Sry-type HMG box 9 and α1(II) collagen mRNA expression levels were significantly enhanced in both cells after 2- and 4-h cyclic tensile strain (5%) treatment.
Conclusions
Medial meniscal root and horn cells have distinct morphologies, reactions to mechanical stress, and cellular phenotypes. Our results suggest that physiological tensile strain is important to activate extracellular matrix production in horn cells.
Note
This fulltext is available in March. 2021.
Published Date
2020-03-26
Publication Title
Journal of Orthopaedic Science
Publisher
Elsevier
ISSN
09492658
NCID
AA11052566
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© 2020 The Japanese Orthopaedic Association.
File Version
author
PubMed ID
DOI
Related Url
isVersionOf https://doi.org/10.1016/j.jos.2020.02.015
License
https://creativecommons.org/licenses/by-nc-nd/4.0/