MMB_1236_73.pdf 1.61 MB
Chiba, Sotaro Institute of Plant Science and Resources (IPSR)Okayama University
Endogenous non-retroviral RNA like sequences (NRVSs) have been discovered in the genome of a wide range of eukaryotes. These are considered as fossil RNA viral elements integrated into host genomes by as-yet-known mechanisms, and in many cases, those fossils are estimated to be millions-of-years-old. It is likely that the number of NRVS records will increase rapidly due to the growing availability of whole-genome sequences for many kinds of eukaryotes. Discovery of the novel NRVSs and understanding of their phylogenetic relationship with modern viral relatives provide important information on deep evolutionary history of RNA virus-host interactions. In this chapter, therefore, the common strategies for the identification and characterization of endogenous NRVSs from plants, insects, and fungi are described.
Molecular fossil record
Methods in Molecular Biology v.1236
Plant Virology Protocols
Kondo H., Chiba S., Suzuki N. (2015) Detection and Analysis of Non-retroviral RNA Virus-Like Elements in Plant, Fungal, and Insect Genomes. In: Uyeda I., Masuta C. (eds) Plant Virology Protocols. Methods in Molecular Biology (Methods and Protocols), vol 1236. Humana Press, New York, NY
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