ID 57359
FullText URL
Author
Uchiyama, Hironobu NODAI Genome Research Center, Tokyo University of Agriculture
Sasaki, Ken Graduate School of Agriculture, Tamagawa University
Hinosawa, Shogo Graduate School of Agriculture, Tamagawa University
Tanaka, Keisuke NODAI Genome Research Center, Tokyo University of Agriculture
Matsumura, Kentarou Graduate School of Environmental and Life Science, Okayama University
Yajima, Shunsuke Graduate School of Environmental and Life Science, Okayama University
Miyatake, Takahisa Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID researchmap
Abstract
The molecular basis of death feigning, an antipredator behavior that has received much attention recently, was analyzed. We compared the gene expression profiles of strains with different behaviors, i.e., different durations of death feigning, in the beetle Tribolium castaneum. Beetles artificially selected for short (S) and long (L) durations of death feigning for many generations were compared thoroughly by RNA sequencing. We identified 518 differentially expressed genes (DEGs) between the strains. The strains also showed divergence in unexpected gene expression regions. As expected from previous physiological studies, genes associated with the metabolic pathways of tyrosine, a precursor of dopamine, were differentially expressed between the S and L strains; these enzyme-encoding genes were expressed at higher levels in the L strain than in the S strain. We also found that several genes associated with insulin signaling were expressed at higher levels in the S strain than in the L strain. Quantitative real-time PCR analysis showed that the relative expression levels of Tchpd (encoding 4-hydroxyphenylpyruvate dioxygenase, Hpd) and Tcnat (encoding N-acetyltransferase, Nat) were significantly higher in the L strain than in the S strain, suggesting the influence of these enzymes on the supply of dopamine and duration of death feigning.
Published Date
2019-09-30
Publication Title
Scientific reports
Volume
volume9
Issue
issue1
Publisher
Springer Nature
Start Page
14001
ISSN
20452322
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© The Author(s) 2019
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PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1038/s41598-019-50440-5
License
http://creativecommons.org/licenses/by/4.0/
Citation
Uchiyama, H., Sasaki, K., Hinosawa, S. et al. Transcriptomic comparison between beetle strains selected for short and long durations of death feigning. Sci Rep 9, 14001 (2019) doi:10.1038/s41598-019-50440-5
Funder Name
Japan Society for the Promotion of Science
助成番号
26291091
18H02510