ID 56312
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Nishimura, Noriyuki Radiation Breeding Division, Institute of Crop Science, National Agriculture and Food Research Organization
Tsuchiya, Wataru Structural Biology Team, Advanced Analysis Center, National Agriculture and Food Research Organization
Moresco, James J. Department of Molecular Medicine, The Scripps Research Institute
Hayashi, Yuki Division of Biological Science, Graduate School of Science, Nagoya University
Satoh, Kouji Radiation Breeding Division, Institute of Crop Science, National Agriculture and Food Research Organization
Kaiwa, Nahomi Radiation Breeding Division, Institute of Crop Science, National Agriculture and Food Research Organization
Irisa, Tomoko Radiation Breeding Division, Institute of Crop Science, National Agriculture and Food Research Organization
Kinoshita, Toshinori Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University
Schroeder, Julian I. Division of Biological Sciences, Cell and Developmental Biology Section, University of California
Yates III, John R. Department of Molecular Medicine, The Scripps Research Institute
Hirayama, Takashi Institute of Plant Science and Resources, Okayama University
Yamazaki, Toshimasa Structural Biology Team, Advanced Analysis Center, National Agriculture and Food Research Organization
Abstract
Abscisic acid (ABA) regulates abiotic stress and developmental responses including regulation of seed dormancy to prevent seeds from germinating under unfavorable environmental conditions. ABA HYPERSENSITIVE GERMINATION1 (AHG1) encoding a type 2C protein phosphatase (PP2C) is a central negative regulator of ABA response in germination; however, the molecular function and regulation of AHG1 remain elusive. Here we report that AHG1 interacts with DELAY OF GERMINATION1 (DOG1), which is a pivotal positive regulator in seed dormancy. DOG1 acts upstream of AHG1 and impairs the PP2C activity of AHG1 in vitro. Furthermore, DOG1 has the ability to bind heme. Binding of DOG1 to AHG1 and heme are independent processes, but both are essential for DOG1 function in vivo. Our study demonstrates that AHG1 and DOG1 constitute an important regulatory system for seed dormancy and germination by integrating multiple environmental signals, in parallel with the PYL/RCAR ABA receptor-mediated regulatory system.
Published Date
2018-06-06
Publication Title
Nature Communications
Volume
volume9
Publisher
Springer Nature
Start Page
2132
ISSN
2041-1723
NCID
AA12645905
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
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publisher
PubMed ID
DOI
Related Url
isVersionOf https://doi.org/10.1038/s41467-018-04437-9