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ID 52805
FullText URL
Author
Sato, Kota
Morimoto, Nobutoshi
Kurata, Tomoko
Mimoto, Takafumi
Miyazaki, Kazunori
Ikeda, Yoshio
Abstract
We have recently reported spinal blood flow-metabolism uncoupling in an amyotrophic lateral sclerosis (ALS) animal model using Cu/Zn-superoxide dismutase 1 (SOD1)-transgenic (Tg) mice, suggesting a relative hypoxia in the spinal cord. However, the hypoxic stress sensor pathway has not been well studied in ALS. Here, we examined temporal and spatial changes of the hypoxic stress sensor proteins HIF-1 alpha and its downstream proteins (VEGF, HO-1, and EPO) during the normcodccourse of motor neuron (MN) degeneration in the spinal cord of these ALS model mice. We found that HIP-1 alpha protein expression progressively increased both in the anterior large MNs and the surrounding glial cells in Tg mice from early symptomatic 14 week (W) and end stage 18W. Double immunofluorescence analysis revealed that HIP-1 alpha, plus GFAP and Iba-1 double-positive surrounding glial cells, progressively increased from 14 W to 18 W, although the immunohistochemistiy in large MNs did not change. Expression levels of VEGF and HO-1 also showed a progressive increase but were significant only in the surrounding glial cells at 18W. In contrast, EPO protein expression was decreased in the surrounding glial cells of Tg mice at 18W. Because HIF1-alpha serves as an important mediator of the hypoxic response, these findings indicate that MNs lack the neuroprotective response to hypoxic stress through the HIF-1 alpha system, which could be an important mechanism of neurodegeneration in ALS.
Keywords
ALS
HIF-1 alpha
VEGF
HO-1
EPO
Published Date
2012-09-14
Publication Title
Brain Research
Volume
volume1473
Publisher
Elsevier Science Bv
Start Page
55
End Page
62
ISSN
0006-8993
Content Type
Journal Article
Related Url
http://ousar.lib.okayama-u.ac.jp/metadata/52529
language
英語
Copyright Holders
(c) 2012 Elsevier B.V. All rights reserved.
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author
Refereed
True
DOI
Web of Sience KeyUT