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ID 51442
FullText URL
Author
Naito, H.
Danura, T.
Kass, I. S.
Morita, K.
Abstract
Rats were subjected to 90 min of focal ischemia by occluding the left middle cerebral and both common carotid arteries. The dynamic changes in the formation of brain ischemic areas were analyzed by measuring the direct current (DC) potential and reduced nicotinamide adenine dinucleotide (NADH) fluorescence with ultraviolet irradiation. In the lidocaine group (n = 10), 30 min before ischemia, an intravenous bolus (1.5 mg/kg) of lidocaine was administered, followed by a continuous infusion (2 mg/kg/h) for 150 min. In the control group (n = 10), an equivalent amount of saline was administered. Following the initiation of ischemia, an area of high-intensity NADH fluorescence rapidly developed in the middle cerebral artery territory in both groups and the DC potential in this area showed ischemic depolarization. An increase in NADH fluorescence closely correlated with the DC depolarization. The blood flow in the marginal zone of both groups showed a similar decrease. Five minutes after the onset of ischemia, the area of high-intensity NADH fluorescence was significantly smaller in the lidocaine group (67% of the control; P = 0.01). This was likely due to the suppression of ischemic depolarization by blockage of voltage-dependent sodium channels with lidocaine. Although lidocaine administration did not attenuate the number of pen-infarct depolarizations during ischemia, the high-intensity area and infarct volume were significantly smaller in the lidocaine group both at the end of ischemia (78% of the control; P = 0.046) and 24 h later (P = 0.02). A logistic regression analysis demonstrated a relationship between the duration of ischemic depolarization and histologic damage and revealed that lidocaine administration did not attenuate neuronal damage when the duration of depolarization was identical. These findings indicate that the mechanism by which lidocaine decreases infarct volume is primarily through a reduction of the brain area undergoing NADH fluorescence increases which closely correlates with depolarization.
Keywords
hypoxia
infarction
lidocaine
NADH fluorescence
DC potential
depolarization
Published Date
2013-04-03
Publication Title
Neuroscience
Volume
volume235
Start Page
59
End Page
69
ISSN
0306-4522
Content Type
Journal Article
Official Url
http://dx.doi.org/10.1016/j.neuroscience.2013.01.010
Related Url
http://ousar.lib.okayama-u.ac.jp/metadata/51452
language
英語
Copyright Holders
(C) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.
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Refereed
True
DOI
Web of Sience KeyUT