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ID 47455
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Author
Umena, Yasufumi
Kawakami, Keisuke
Kamiya, Nobuo
Abstract
Photosystem II is the site of photosynthetic water oxidation and contains 20 subunits with a total molecular mass of 350 kDa. The structure of photosystem II has been reported at resolutions from 3.8 to 2.9 angstrom. These resolutions have provided much information on the arrangement of protein subunits and cofactors but are insufficient to reveal the detailed structure of the catalytic centre of water splitting. Here we report the crystal structure of photosystem II at a resolution of 1.9 angstrom. From our electron density map, we located all of the metal atoms of the Mn(4)CaO(5) cluster, together with all of their ligands. We found that five oxygen atoms served as oxo bridges linking the five metal atoms, and that four water molecules were bound to the Mn(4)CaO(5) cluster; some of them may therefore serve as substrates for dioxygen formation. We identified more than 1,300 water molecules in each photosystem II monomer. Some of them formed extensive hydrogen-bonding networks that may serve as channels for protons, water or oxygen molecules. The determination of the high-resolution structure of photosystem II will allow us to analyse and understand its functions in great detail.
Published Date
2011-05-05
Publication Title
Nature
Volume
volume473
Issue
issue7345
Publisher
Nature Publishing Group
Start Page
55
End Page
60
ISSN
0028-0836
NCID
AA00752384
Content Type
Journal Article
Project
Research Center of New Functional Materials for Energy Production, Storage, and Transport
language
英語
Copyright Holders
©2011 Macmillan Publishers Limited. All rights reserved
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Refereed
True
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