ID 47262
JaLCDOI
FullText URL
Author
Terada, Chuji
Yoshida, Aki
Nasu, Yoshihisa
Mori, Shuji
Tomono, Yasuko
Takahashi, Hideo K.
Ozaki, Toshifumi Kaken ID researchmap
Nishida, Keiichiro Kaken ID researchmap
Abstract
We investigated the expression and localization of high-mobility group box chromosomal protein-1 (HMGB-1) in human osteoarthritic (OA) cartilage in relation to the histopathological grade of cartilage destruction, and examined the role of HMGB-1 in the regulation of proinflammatory cytokine expression in chondrocytes. An immunohistochemical study demonstrated that total HMGB-1-positive cell ratios increase as the Osteoarthritis Research Society International (OARSI) histological grade increased. The population of cytoplasmic HMGB-1-positive chondrocytes was especially increased in the deep layers of higher-grade cartilage. The ratios and localization of receptors for advanced glycation end products (RAGE) expression by chondrocytes in Grade 2, 3, and 4 were significantly higher than those in Grade 1. In vitro stimulation with IL-1β, but not TNFα, significantly upregulated the expression of HMGB-1 mRNA by human OA chondrocytes. Both IL-1β and TNFα promoted the translocation of HMGB-1 from nuclei to cytoplasm. IL-1β and TNFα secretions were stimulated at higher levels of HMGB-1. The results of our study suggest the involvement of HMGB-1 in the pathogenesis of cartilage destruction in OA.
Keywords
HMGB-1
RAGE
chondrocyte
osteoarthritis
cartilage
Amo Type
Original Article
Published Date
2011-12
Publication Title
Acta Medica Okayama
Volume
volume65
Issue
issue6
Publisher
Okayama University Medical School
Start Page
369
End Page
377
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
Copyright Holders
CopyrightⒸ 2011 by Okayama University Medical School
File Version
publisher
Refereed
True
PubMed ID
Web of Science KeyUT