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ID 42487
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Title Alternative
Studies on the Glutamic Acid Metabolism of Bacteria I: Glutamic acid metabolism of Escherichia coli communis and its application to the analysis of glutamic acid
Author
Akita, Yoshimi
Abstract
It is well known that glutamic acid, as well as aspartic acid, plays an important role in the metabolism of microorganisms. The author performed many experiments in order to study the physiological aspects of E. coli communis from the stand point of glutamic acid metabolism, and to apply it to the analysis of glutamic acid. The results were as follows: 1) E. coli can grow at any pH within the range from 5.4 to 8.0, but the growth is the best at pH 6.8 to 7.0. 2) The more remote from the optimum the cultural pH is, the nearer it comes to the optimum after the growth. This phenomenon is particularly remarkable on the acid side. 3) Using glutamic acid as substrate, the decarboxylation is chiefly carried out on the acid side, the deamination on the neutral or slightly alkaline side, and these two reactions are carried out simultaneously at pH 5.5 to 6.5. As for the optimum pH, pH 5.0 for decarboxylation and 7.0 for deamination. 4) The lower the cultural pH is, the higher the glutamic decarboxylase activity of the cultured organism becomes. The optimum reaction pH is, however, never shifted. 5) The E. coli, which was cultured in the pyridoxin-containing semi-synthetic medium, shows a very high activity to glutamic acid, but not to α-ketoglutaric acid, aspartic acid, alanine and pyruvic acid. 6) The aceton powder of the E. coli, which was cultured in the pyridoxin-containing semi-synthetic medium, still has the high glutamic decaboxylase activity. The optimum pH is from 4.4 to 5.1, where carbon dioxide is evolved quantitatively.
Published Date
1957-02-28
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume69
Issue
issue2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
541
End Page
547
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/69/2/69_2_541/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
Copyright© 岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma