ID 33002
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Author
Ouchida, Mamoru Kakenhi
Shigematsu, Hisayuki
Tanaka, Noriyoshi
Kobayashi, Kazuyasu
Inaki, Yasuhiko
Toyooka, Shinichi
Shimizu, Nobuyoshi
Shimizu, Kenji Kakenhi
Abstract
In an attempt to identify tumor suppressor genes on chromosome 10 in non-small cell lung cancers, we isolated 10 types of splicing variants of the HELLS/ SMARCA6 gene transcripts. HELLS/SMARCA6 is a novel member of SNF2 family, which is implicated in cellular function like chromatin remodeling. Variant 1 was an alternatively spliced isoform containing an insertion of a 44-ntd intronic sequence between exons 3 and 4, giving rise to a premature termination of translation. The expression of the variant 1 was detected exclusively in the lung cancer specimens (11 of 43 cases, 26%), but was not detected in corresponding normal tissues. D10S520 marker in the proximity of the HELLS/SMARCA6 gene showed prevalent allelic loss (41%) as compared with flanking markers (25-31%). These results suggest that loss of function of HELLS/SMARCA6 by allelic loss and aberrant proteins by tumor-specific exon creation may result in epigenetic deregulation, leading the lung cells to malignancy or its progression.
Keywords
alternative splicing
HELLS
loss of heterozygosity
lung cancer
SMARCA6
Note
Digital Object Identifer:10.1002/ijc.20407
Published with permission from the copyright holder. This is the author's copy, as published in the Journal of International Journal of Cancer, October 2004, Volume 112, Issue 1, Pages 8-13.
Publisher URL:http://dx.doi.org/10.1002/ijc.20407
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Copyright © 2004 Wiley-Liss, Inc. All rights reserved.
Published Date
2004-10-20
Publication Title
International Journal of Cancer
Volume
volume112
Issue
issue1
Publisher
Wiley-Liss, Inc.
Start Page
8
End Page
13
ISSN
0020-7136
NCID
AA00680002
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
Wiley-Liss, Inc.
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