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ID 2427
Eprint ID
2427
FullText URL
Thumnail K001694.pdf 170 KB
Title Alternative
リポソームを応用した経口ワクチンの開発:経口投与可能なリポソームの脂質組成の検討ならびに経口投与によるIgA 抗体の誘導
Author
Han, Mei
Abstract
n order to evaluate the usefulness of liposomes as oral vaccines, the stability of liposomes and serum IgA antibody response to antigen associated with liposomes after oral administration were examined. Liposomes composed of dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylserine (DPPS), and cholesterol (Chol) (1:1:2, molar ratio), distearoylphosphatidylcholine (DSPC) and Chol (7:2, molar ratio), and DSPC, DPPS, and Chol (7:3:2 or 1:1:2, molar ratio) were stable in acidic solution (pH 2.0), bile, and pancreatin solution, whereas liposomes composed of DPPC and Chol (7:2, molar ratio) and DPPC, DPPS, and Chol (7:3:2, molar ratio) were unstable in pH 2.0 and/or bile solutions. After the oral immunization of antigen (ganglioside GM1)-containing liposomes composed of DPPC, DPPS, and Chol (1:1:2, molar ratio) to mice, the serum IgA antibody responses against ganglioside GM1 were found. Furthermore, when monophosphoryl lipid A was incorporated into liposomes containing ganglioside GM1, further augmentation of IgA responses to ganglioside GM1 was observed. On the other hand, the oral administration with liposomes composed of DPPC, Chol, and ganglioside GM1 (unstable liposomes), ganglioside GM1 mixed with liposomes composed of DPPC, DPPS and Chol, and ganglioside GM1 alone was unable to induce any detectable anti-ganglioside GM1 IgA antibody responses. These results suggest that liposomes which showed the stability to acidic solution, bile, and pancreatin solution would serve effectively as an oral delivery vehicle for inducing mucosal immune responses.
Keywords
liposome
mucosal immunity
oral administration
oral vaccine
stability
Note
掲載順位 69 ; http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9450240&dopt=Abstract
Published Date
1998-03-25
Publication Title
Content Type
Thesis or Dissertation
Grant Number
甲第1694号
Granted Date
1998-03-25
Thesis Type
博士(医学)
Grantor
岡山大学
Official Url
http://dx.doi.org/10.1292/jvms.59.1109
language
日本語
File Version
none
Refereed
Unknown