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ID 19812
Eprint ID
19812
FullText URL
Thumnail 68_1705.pdf 906 KB
Title Alternative
Permeability Studies on Blood Cells by Supravital Stain Ⅰ. Supravital Stain of Leucocyte and Reticulocyte and the Hydrogen Ion Concentration of Media
Author
Oguchi, Motomitsu
Abstract
Supravital staining of granulocyte, lymphocyte and reticulocyte was carried out with a series of dyes including both acidie and basic ones. On each dye the affinity to cells was observed in those media which pH were changed serially from 1 to 13 using Mcllvaine's or Sorensen’s buffer solution. On the other hand the increasing or decreasing effect of hydrogen ion concentration of media on the charges of dyes was observed electrophoretically. In general, strongly charged dyes, both positive and negative, failed to invade into cells in the whole range of pH, while the Weakly charged dyes showed a positive stain in a certain region of pH, i. e. the acidic dyes in the acidic media and hasic dyes in the basic media, showing the accelerated permeability of dyes to cells in their fairly discharged states. However, some dyes proved to be inpermeable even in their weakly charged states. This inpermeability was attributed to the large molecnlar weight of the dyes or to the sparing solubility to water, alcohol, ether and ether-alcohol.
Published Date
1956-10-31
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume68
Issue
issue10
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
1705
End Page
1719
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/68/10/68_10_1705/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma