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ID 19758
Eprint ID
19758
FullText URL
Title Alternative
Separation of paripheral leukocytes by counterflow centrifugation elutriation
Author
Sudo, Michiyasu
Matsubara, Fumie
Araki, Hiroyuki
Kitani, Hikaru
Tanizaki, Yoshiro
Abstract
JE-6B rotor(Beckman)を用いてcounterflow centrifugation elutriationによる末梢血白血球の分離を試み,若干の検討を加えた。1.flow rateが低い場合はリンパ球が大部分を占めたが,flow rateの増加とともに頻度は減少した。2. flow rateの増加とともに,好塩基球の頻度が増加し,9-10ml/minでは頻度のみでなく細胞数の増加も認められた。各fractionにおけるヒスタミン含有量も同様の傾向が認められた。3.好中球のpeakはflow rate 13ml/minであり以後減少を示した。4.最終段階で好酸球が増加傾向を示しChamber内で最も高値を示した。以上の結果よりcounterflow centrifugation elutriationによる末梢血白血球細胞分離は,in vitroにおける実験に有用な手段である可能性が示唆された。
Abstract Alternative
Separation of peripheral leukocytes was carried out in 5 healthy subjects by counterflow centrifugation elutriation with JE-6B rotor. 1. Number of lymphocytes was predominant at low flow rate (4.5ml/min) of counterflow centrifugation elutriation. 2. As flow rate was increased (9-10ml/min), the purity and number of basophils, and histamine content in the fraction were gradually increased. 3. The purity and number of neutrophils were gradually increased, and the purity was reached the peak at 13ml/min. 4. The purity and number of eosinophils were gradually increased at the late stage of the centrifugation elutriation. The results obtained here might suggest that cell separation by counterflow centrifugation elutriation is more useful for experiments, especially allergy testing, in vitro.
Keywords
逆流負荷遠心法 (Counterflow centrifugation elutriation)
JE-6Bローター (JE-6B rotor)
細胞分離 (Cell separation)
好塩基球 (Basophils)
Published Date
1988-08
Publication Title
環境病態研報告
Publication Title Alternative
Papers of the Institute for Environmental Medicine, Okayama University Medical Scool
Volume
volume59
Publisher
岡山大学医学部附属環境病態研究施設, 岡山大学医学部附属病院三朝分院
Publisher Alternative
Institute for Environmental Medicine, Okayama University Medical Scool
Start Page
37
End Page
41
ISSN
09133771
NCID
AN10084718
Content Type
Departmental Bulletin Paper
language
日本語
File Version
publisher
Refereed
False
Eprints Journal Name
mmc