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ID 19335
Eprint ID
19335
FullText URL
70_4225.pdf 1.43 MB
Title Alternative
On the Effects of Temperatures upon Enzyme Activity
Author
Kanda, Mizuho
Abstract
In order to experiment the effects of low and high temperature upon the enzyme activity, the author used papayotin and taka-diastase and he came to the following conclusions: 1) In refregirathing experiment in 0℃, -18℃, -48℃ and -52℃, optimum pH in the activity of papayotin and taka-diastase is found 4.6~5.0. 2) The activity of papayotin and taka-diastase is not reduced even when the enzyme is iced in 0℃ for ten days. 3) After refregirating just papayotin powder in -18℃ and working it on gelatin solution, the enzyme activity increases; and on the contrary, after refregirating gelatin solution in -18℃ and working it on the papayotin powder, the enzyme activity decreases; and lastly, it is found out that the influence of refregirating on the enzyme activity is stronger when refregirated in -18℃ for two hours han only thirty minutes in the same degree of temperature. In the experiment used taka-diastase, when either just enzyme powder, or starch solution, or mixed solution of enzyme powder and buffer solution or of enzyme powder, starch solution and buffer solution are iced in -18℃ the enzyme activity has tendency to increase a little. In case of refregirating just starch solution, the increase of taka-diastase is seen very clearly; and two hours' refregirating in -18℃ is much stronger in activity of increase than of thirty minutes. 4) In the experiment in -48℃, papayotin iced for thirty minutes and worked upon gelatin solution shows a little increase in its activity; but in the case when just gelatin solution is iced, it is not clear of any influence of refregirating. In the same experiment, when either or taka-diastase or starch solution or mixed solution of these two is iced in -48℃, the activity of taka-diastase begins to increase a little and stronger increase is found always in just starch solution which is iced for two hours rather than thirty minutes. 5) In the experiment is -52℃, just papayotin is iced and its activity with a little increase is more clealy shown in thirty minutes' refregirating rather than that of one or two hours. Either just starch solution or mixed solution of taka-diastase and starch solution is iced, the influence of refregirating on the activity of papayotin is not clear. In the experiment used take-diastase, its activity increases when just starch solution is iced, and on the other hand, the influence of refregirating upon the activity is not clear when either of taka-diastase or mixed solution of taka-diastase and starch solution is iced. 6) In short, it is found out that the papayotin in the decomposition of gelatin increases when the papayotin powder is iced; and taka-diastase in the decomposition of starch increases when the starch solution is iced: the former is the increase of papayotin based on the disaggregation of colloidal granule (F, Nord) and the latter is that the activity of taka-diastase could be made easier by refregirating starch solution and making granule into smaller pieces. 7) In heating take-diastase solution in 70℃ for thirty minutes, the influence of heat upon the activity of taka-diastase is not shown; but the slight decrease of activity would be seen in case of heating in 80℃ for thirty minutes. 8) In heating taka-diastase solution in 100℃ for thirty minutes, the activity is gone completely which will never come back even when taka-diastase is iced in -18℃.
Published Date
1958-11-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume70
Issue
issue11
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
4225
End Page
4244
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/70/11/70_11_4225/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma