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ID 19278
Eprint ID
19278
FullText URL
Thumnail 71_1385.pdf 1.95 MB
Title Alternative
Studies on the Bone-Marrow Tissue Culture by the Roller-Tube Method. Part 3. The Effects of Sera and Ascites on the Tissue Culture
Author
Sugano, Takashi
Abstract
After determining the optimal concentration of serum, the transudate and exudate of ascites in the abdominal cavity as the culture media in the rabbit bone-marrow tissue culture by the roller-tube method, the auther compared the effects of these three media on tissue culture: 1. As for serum the concentration at 20 per cent shows the best relative tissue growth rate and the highest wandering velocity of pseudoeosinophils and the least regressive degeneration, and therefore, this at 20 per cent concentration seems to be most suitable as the culture medium. Moreover, when the effect of the medium containing this 20% serum is compared with that of the control (the culture medium without serum), the former excels the latter in the effectiveness in every respects such as the relative tissue growth rate, the wandering velocity of pseudoeosinophils and the appearance of regressively degenerated granules. 2. As for the medium containing the transudate and exudate of the abdominal cavity, the rlative tissue growth rate in both cases is in an inverse proportion to the concentration, but the appearance of regressively degenerated granules is least at the concentration of 30 per cent, and cells are preserved in a good condition, proving that 30 per cent of the transudate or exudate of the abdominal cavity contained in the culture medium is most effective. Furthermore, in the comparison of the medium containing 30 per cent of either one of the two no great difference can be recognized, but the medium containing exudate is somewhat superior. When the effects of the medium containing transudate or exudate are compared with that of the control (the medium without either the transudate or exudate), the former clearly excels the latter in every respects such as in the relative tissue growth rate, the wandering velocity of pseudoeosinophils and the appearance of regressively degenerated granules. 3. On comparing the case of the medium containing serum with that containing the transudate or exudate of the abdominal cavity, the great difference can not be observed in the wandering velocity of pseudoeosinophils in all three cases, but in the case of the medium containing serum the relative tissue growth rate is greatest and also the appearance of regressively degenerated granules is least of all. Therefore, the former is fairly superior to either the medium containing the transudate or the medium containing exudate. 4. From these findings it seems that the medium containing the 20% serum, 15% chick-embryo extracts and 65% Gey's solution seems to be most suitable for the tissue culture by the roller-tube method. However, it is believed that the medium containing the 30% ascites, the 15% chick-embryo extracts and 55% Gey's solution can also be used to take the place of the above mentioned medium.
Published Date
1959-03-15
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume71
Issue
issue3-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
1385
End Page
1403
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/71/3-2/71_3-2_1385/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma