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ID 19210
Eprint ID
19210
FullText URL
71_879.pdf 1.93 MB
Title Alternative
Studies on Reticulocyte Ripening Subsuances Part 3. The cellulor Origin and the characterisbics of the Ripening Substance
Author
Sanada, Hiroshi
Abstract
From the results presented in Part 2 it is supposed that the reticulocyte ripening substances in the bovine liver found by the author may be protein belonging to a globulin fraction or a substance attached to some particles of the cytoplasm, which can not be precipitated by the general centrifugation. The supernatant obtained from the saline extract by centrifuging at a low speed was again put to ultra-centrigu-gation at 40,000 r. p. m., thus splitting fraction into two, soluble fractions and microsome or mitochondrial fractions. Ripening test on reticulocytes proved that the effective substance had been transfered to the soluble fraction. The globulin fraction obtained from the saline exfract by the half saturation with ammonium sulfate also proved to be effective. Electro-paper chromatography of the protein obtained from the saline extract revealed that it contained a fraction of γ-globulin which was found to be lacking in the water extract. Thus the ripening substance of reticulocyte has been prove to be a protein belonging to the γ-globulin fraction originated from the hyaloplasm.
Published Date
1959-02-28
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume71
Issue
issue2-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
879
End Page
883
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/71/2-2/71_2-2_879/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma