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ID 19153
Eprint ID
19153
FullText URL
Thumnail 71_659.pdf 453 KB
Title Alternative
Studies on the Metabolic Aspects of Mycobacterium tuberculosis Ⅰ: Cell-Free Extract, its Outer and Inner Solutions of Dialysis and Their Dehydrogenae Activities
Author
Takizawa, Sennosuke
Abstract
The acid-fast lipid of Mycobacterium tuberculosis, which is considered to be important for the manifestation of its virulence, has very intimate relation with the concentration of glycerol in culture media. In addition to this, glycerol plays a very important role in the metabolism of carbon sources of Mycobacterium tuberculosis. In consideration of these facts, the author studied the dehydrogenase activities of the cell-free extract and of its outer and inner solutions of the dialyzate of Mycobacterium tuberculosis cultured in each of Sauton's liquid media containing glycerol and glucose in place of glycerol. The results are briefly summarized as follows: I) The dehydrogenase of the cell-free extract of H37Rv and H37Ra, particularly of H37Rv, cultured in Sauton's glycerol media are stronger than those cultured in glucose media. 2) For the dehydrogenation of lactate and succinate by the cell-free extract, the addition of the outer solution of dialysis is not needed. In cases of malate and histidine, however, the outer solution is highly needed. 3) The lactic dehydrogenase of the cell-free extract obtained by disintegration in the mortar and centrifugation is very unstable to heating.
Published Date
1959-02-28
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume71
Issue
issue2-1
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
659
End Page
665
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/71/2-1/71_2-1_659/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma