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ID 19145
Eprint ID
19145
FullText URL
Thumnail 71_651.pdf 3.77 MB
Title Alternative
Studies on Urobilinogen Part 2. Separation of the coloured substances of stercobilinogen and mesobilinogen with the ehrlich's aldehyde reagent by the chromatography
Author
Sakurai, Hiroshi
Abstract
The paper chromatography was attempted for the separation of stercobilinogen, predominating in urobilinogen of living body, and mesobilinogen, being supposed to partly exist in vivo, on the coloured substances of them by the addition of the Ehrlich's aldehyde reagent, and the columnchromatography with the column of a cellulose powder was also attempted for the separation of the urobilin coexisting in the coloured substances of urobilinogen by the addition of the Ehrlich's aldehyde reagent. And the results are as follows. 1. The coloured substance of urobilinogen and urobilin are definitely separated, but the separation between the coloured substances of stercobilinogen and mesobilinogen by the addition of the Ehrlich's aldehyde reagent is impossible, by the paper chromatography with the developing solvents of a methanol and water mixture (1:2), a methanol, butanol and ammouia mixture (1:2:1), a 28 % amonia and butanol mixture (4:96), N-Ammonia-condensed butanol, 10 % Ammoniacondensed butanol, and a butanol, and a butanol, propanol and ammonia mixture (2:2:1) etc. 2. The coloured substances of stercobilinogen and mesobilinogen with the Ehrlich'c aldehyde reagent display the Rf 0,81 and 0. 64 respectively and are definitely separated, on the use of the paper with the stationary phase made with glacial acetic acid and, with the developing solvents of a methanol, ammonia and water mixture (76:4:20) and a methanol, butanol and ammonia mixture (2:2:1). 3. The separation of stercobilin and urobilin IX α are easily obtained by the paper chromatography with the developing solvents of a methanol, ammonia and water mixture (76:4:20), and a methanol, butanol and ammonia mixture (2:2:1). 4. The coloured substances of urobilinogen with the Ehrlich's aldehyde reagent and urobilin in them can be separated by the column chromatography with the column of cellulose powder and the developing solvent of a methanol and water mixture (1:2). Therefore, as the absorption curve of the coloured substance with the Ehrlich's aldehyde reagent displays having only one absorption maximum at 558 mμ, it must he definitely distinguished from the urobilin which becomes coexisted during the process.
Published Date
1959-02-28
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume71
Issue
issue2-1
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
651
End Page
658
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/71/2-1/71_2-1_651/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma