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ID 19062
Eprint ID
19062
FullText URL
Thumnail 71_327.pdf 1.14 MB
Title Alternative
Studies on Serum Lipoprotein 2. Electropaperchromatography of lipoprotein especially the masked lipid
Author
Tokura, Mataharu
Abstract
By using the apparatus demonstrated in the first report, the author has studied the lipoprotein of serum, extending at 110 v. for 7 hours filling the space with air and staining with Sudan black B (S. B. B). The following results have been obtained. 1. From the integrated values of the area obtained by densitometry on the papers stained with S. B. B it is possible to know roughly the amount of lipoprotein of serum and exact quantitative relation between α and β lipoproteins. 2. The drying of paper causes the masking of the lipids of lipoprotein which can be unmasked again by heating at 110℃. for 20 minutes. 3. Pepsin, phenol and some carboxylic acids act as to unmask the masked lipoprotein. After unmasking some lipids escape into the solvent during the S. B. B staining in α-lipoprotein, but not in β-lipoprotein. 4. From the observations on the masking and unmasking of serum lipoproteins the binding modus between lipid and protein and the masking-unmasking mechanisms have been discussed.
Published Date
1959-02-28
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume71
Issue
issue2-1
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
327
End Page
332
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/71/2-1/71_2-1_327/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma