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ID 19004
Eprint ID
19004
FullText URL
Thumnail 71_89.pdf 271 KB
Title Alternative
Studies on the Synthetic Media or Culture of some Bacteria Part Ⅲ Study of the metabolism of the bacteria cultured by serial trnsfer on the synthetic fluid media
Author
Nakanishi, Hirotaka
Abstract
Using eleven species of bacteria described in the preceeding paper, the author observed the fluctuation of the growth of bacteria cultured by serial transfer through ten generations on the synthetic fluid media prepared carefully its amino nitrogen contents, and investigated the fluctuation of the enzyme activity of bacteria. 1) The all species of bacteria except Staphylococcus could be cultured succesfully through ten generations on the synthetic fluid media contained simply few species of amino acids. Moreover, the cellular amount yielded was fairly abundant, so it was suitable preparation for study of its metabolism. It was observed on Sal. enteritidis and Sal. typhi murium that the growth of bacteria increased with the progress of transfers. 2) In the light of O(2)-uptake by Warburg's manometer, the author compared the enzyme activity to four substrates, i.e. glucose, lactic acid, succinic acid and glutamic acid, of the bacteria cultured in the synthetic fluid media by serial trasfers with that of the bacteria cultured normally. Generally, that activity of the former was reduced than that of the latter. But the activities of Sal. typhi 57 S and Shigella flexineri 2 a to glucose and that of Shigella sonnei to glucose and glutamic acid were risen.
Published Date
1959-01-31
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume71
Issue
issue1
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
89
End Page
92
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/71/1/71_1_89/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma