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ID 18187
Eprint ID
18187
FullText URL
Thumnail 80_1151.pdf 710 KB
Title Alternative
Studies on Tissue Culture of Spleen Chapter Ⅰ Vital Observation of Splenic Reticulnm Cells by Phase Contrast Microscopy
Author
Kibata, Masayoshi
Abstract
Morphology and movement of splenic reticulum cells of the normal adult mouse were observed in the growth zone of tissue culture by phase contrast microscopy and 16 mm micro-cinematography. 1) For the vital observation of these cells, rectangular cover slip method proved satisfactory. 2) In tissue culture of normal mouse spleen, lymphocytes appeared initially untill 24 hours, follwed by some types of reticulum cells which increased after 24 to 48 hours of culture, and finally fibroblasts increased in the growth zone. 3) According to the morphology, function and degree of maturation, these reticulum cells could be classified into three groups; Ⅰ, Ⅱa, Ⅱb, Ⅲa and Ⅲb. There were intermediate types between these cells and type Ⅰ reticulum cells matured into type Ⅱ and Ⅲ reticulum cells. 4) Type Ⅱa reticulum cells possessed multiple pseudopods and showed active migratory movement, their nucleus locating in the forwarding part of the cytoplasm.
Published Date
1968-12-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume80
Issue
issue11-12
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
1151
End Page
1158
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/80/11-12/80_11-12_1151/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma