このエントリーをはてなブックマークに追加
ID 18152
Eprint ID
18152
FullText URL
Thumnail 80_857.pdf 221 KB
Title Alternative
Studies on Adenovirus Type 12 2. On the proliferation of the virus in tissue culture by slant agar and monolayer methods
Author
Fujita, Kozo
Abstract Alternative
In the present experiments minute quantities (10(-4), 10(-5), 10(-6) TCID(50)) of adenovirus type 12were used for the infection of culture cells, HeLa-S. After infecting the cells with virus, the tissue cultures were conducted by the slant agar and monolayer methods, to compare the degree of virus proliferation for successive generations in each experimental group. The results of the study are summarized as follows. 1. When HeLa S(3) cells were infected with minute quantities of virus for successive generations (30 days each generation), the virus proliferation, as determined by cytopathic effect (CPE), was better in the case of the slant agar culture than in the monolayer culture. 2. Similar cultures were conducted by two methods in which the culture period was changed to 7 days or 30-35 days for each generation. As a result it was found that the proliferation of the virus could be detected only at the fifth or sixth generation in all the experimental groups. Both the slant agar culture and the monolayer culture of infected HeLa-S(3) cells gave the results similar to those obtained in the successive cultures conducted by the freezingthawing method (reported in Part 1).
Published Date
1968-08-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume80
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
857
End Page
860
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/80/7-8/80_7-8_857/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma