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ID 17982
Eprint ID
17982
FullText URL
Thumnail 79_259.pdf 606 KB
Title Alternative
Studies on Malic Dehydrogenese Isozyme Part Ⅰ. A Study on Malic Dehydrogenese Activities and Isozymes of Various Human Organs
Author
Takayasu, Masao
Abstract
Malic dehydrogenese (MDH) activities and isozymes of various human organs were measured. The assays of isozyme were carried out by means of agargel electrophoresis. The highest MDH activity was demonstrated in heart muscle. Liver, skeletal muscle, kidney and brain showed considerably high MDH activities. Six fractions were distinguished in many MDH isozymograms. In order of movility towards the cathod, each MDH isozyme was named MD(1), MD(2)…MD(6). Intracellular distribution of each MDH isozyme was investigated by means of cell fractionation, and MD(1)~MD(5) were confirmed mitochondrial, MD(6) cytoplasmic, in origin. MDH isozyme-patterns of human organs were divided into 3 groups according to the ratio of mitochondrial MDH (m-MDH) and cytoplasmic MDH (c-MDH). Group Ⅰ (m-MDH>c-MDH): heart muscle, kidney, skeletal muscle, white blood cell, red blood cell and lung-tissues. Group Ⅱ (m-MDH≒c-MDH): liver, pancreas. Group Ⅲ (This group situates between Group Ⅰ and Group Ⅱ): brain, gastric mucosa, and spleen. Studies on inhibitory effect of p-chlor-mercuri-benzoate (PCMB) on each MDH isozyme and heat stability of each MDH isozyme demonstrated that m-MDH was not inhibited by PCMB and stable to a heat test at 50℃ for 30,' when 1-malate was used as substrate. It was also observed that m-MDH was activated by a high concentration of 1-malate. According to the standard deviation of MD(6) and MD(3) activites, c-MDH considered regulatory enzyme and m-MDH constitutive enzyme, respectively.
Published Date
1967-04-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume79
Issue
issue3-4
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
259
End Page
270
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/79/3-4/79_3-4_259/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma