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ID 17863
Eprint ID
17863
FullText URL
84_397.pdf 447 KB
Title Alternative
A Study on the Methodology of Screenig Tests for Acatalasemic Gene Carrier
Author
Takagoshi, Yoshiaki
Abstract
The way of preserving blood specimens is an important factor on the screening tests for acatalasemic gene carrier. Because, the decay time of catalase activity in blood depends on the physical conditions, largely on temperature, where the blood specimens are kept. The author investigated the decay rate of catalase activity under following conditions. 1. Preservation of human blood in a vacuum flask at the temperature of 0~10℃. 2. Preservation in a deep-freezer at -20℃. In the former condition the blood specimen did not lose catalase activity at least seven days, and afterwards decay went on gradually. On the contrary, in the latter frozen specimens some degree of decay occurred soon. Afterwards, however, they kept constant catalase activity, 84% of the initial value, for months. So, it is concluded that for the practice of screening tests preservation in a vacuum flask at the temperature of 0~10℃ is preferable. Next, the author devised a handy, feasible way of screening tests named the !! floating disk method. !! It is a modification from M. Gagnon's estimation of catalase activity by measuring floating time of a piece of paper disk dipped with enzymatic specimen in hydrogen per-oxide solution. The screening level set up by this method is 12.5cm for the depth and 13.5 sec for the floating time.
Published Date
1972-12-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume84
Issue
issue11-12
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
397
End Page
404
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/84/11-12/84_11-12_397/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma