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ID 17563
Eprint ID
17563
FullText URL
Thumnail 85_183.pdf 735 KB
Title Alternative
Studies on T-antigen of the clones derived from a single cell in SV 40-transformed mouse embryo cells by the immunofluorescent method
Author
Egusa, Kuniyuki
Seki, Shuji
Oda, Takuzo
Abstract
In our previous observation, the T-antigen in unclond mouse embryo cells transformed by SV 40 or SV 40 DNA declined gradually through long term cultivation. To analyze whether this phenomenon is due to selection or reversion and/or conversion, we observed the T-antigen of a series of clones derived from a single cell in the SV 40-transformed cells by the immunofluorescent method. The T-antigen was either positive or negative in all cells in each clone, and none of the clones was consisted of the mixture of T-antigen positive and negative cells. The T-antigen negative cells were more predominant in the rate of growth than the T-antigen positive ones in this mouse embryo cells. One reasonable explanation on the data may be that the decline of T-antigen in the uncloned SV 40-transformed cells is due to the selection of the T-antigen negative cells. This fact may be one of the reason for difficulty in establishing the SV 40-transformed mouse embryo cell lines.
Published Date
1973-06-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume85
Issue
issue5-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
183
End Page
187
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/85/5-6/85_5-6_183/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma