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ID 17442
Eprint ID
17442
FullText URL
Thumnail 87_587.pdf 2.6 MB
Title Alternative
In-vitro Study of Cell-mediated Immunity in Collagen Diseases Part Ⅰ: Macrophage migration inhibition test (MIT) and leucocyte migration inhibition test (LMIT) in systemic lupus erythematosus (SLE)
Author
Yano, Keisuke
Abstract
The appearance of circulating antibodies to nuclear components (e.g. DNA, Histone, Nucleoprotein) is characteristic of SLE and may have pathogenic importance. But, it is not evident why these autoantibodies are produced. Concerning these immunological abnormalities, most research has concentrated on the humoral immune responses made against nuclear antigens. The role of cell-mediated immunity to unclear antigens in SLE has not been investigated. Therefore, the evidence of cell-mediated immunity to native calf thymus DNA was determined by MIT and LMIT in patients with SLE and normal subjects. The mean percent migration of MIT in SLE and normal subjects was 79.8±24.1 and 101.4±15.3%, respectively. The percent migration below 70.8% was considered to be a positive test and 17 out of 42 patients with SLE had a positive test. In contrast, none of normal subjects did. These findings indicate that there is cellulay hypersensitivity to native DNA and peripheral lymphocytes of SLE patients produced a migration inhibitory factor (MIF) with responding to it. No correlation could be made between results of MIT and staining patterns of antinuclear factor (AMF), titers of anti-DNA antibody, serum complement levels and activity of the disease. The results of LMIT was differed from those of MIT. The mean percent migration of LMIT was not significantly different in SLE patients and normal subjects, and only a few cases in SLE exhibited a positive test. Considering the dissociation of these results in MIT and LMIT, the conclusion has been made that MIT is more useful in investigation the existence of cellular hypersensitivity. In addition, the role of T cells in the development of autoimmunity was discussed in accordance with the existence of cellular hypersensitivity to native DNA in SLE.
Published Date
1975-08-31
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume87
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
587
End Page
599
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/87/7-8/87_7-8_587/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma