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ID 17252
Eprint ID
17252
FullText URL
Thumnail 90_313.pdf 7.63 MB
Title Alternative
Morphological changes of tumorigenesis of DMBA-induced rat mammary tumors
Author
Kobuchi, Kinya
Abstract
7,12-Dimethylbenz(a)anthracene (DMBA) 20mg was administered to 55-days-old Sprague-Dawley rats with gastric intubation. Two or three rats were sacrificed daily during the first week following administration of DMBA, then, weekly up to the 20th week. Mammary glands of each different stage were examined by the whole mount method, light microscopy and electron microscopy to observe tumorigenesis of rats mammary tumors introduced by DMBA. 1. On the 4th day following administration of DMBA, mammary epithelial cells at the peripheral portion of the duct demonstrated dilatation of endoplasmic reticulum and Golgi apparatus. On the 2nd week, an evidence of hypersecretion was well established. 2. However, on the 4th week, mammary epithelial cells showed atrophy and their intracellular organelles were regressed. On the other hand, myoepithelial cells showed cytoplasmic extensions to surround mammary epithelial cells. Stroma around the ductules also showed marked proliferation. 3. On the 7th week, mammary glands, examined by the whole mount method, demonstrated hyperplasty to form small nodules, namely, hyperplastic alveolar nodules (HAN). Among some rats, stroma around the ductules kept growing to be fibroadenoma. 4. On the 9th week, a tumor became palpable, and its histology was tubular adenocarcinoma. 5. Electron microscopic findings of carcinoma were increase of nucleocytoplsmic ratio, irregularity of nucleus, hypertrophy of nuclear membrane and enlargement of intracellular space etc..
Published Date
1978-04-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume90
Issue
issue3-4
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
313
End Page
334
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/90/3-4/90_3-4_313/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma