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ID 16944
Eprint ID
16944
FullText URL
Thumnail 94_1003.pdf 793 KB
Title Alternative
Polyacrylamide Gel Electrophoretic Studies of Residual Catalase in Acatalasemia
Author
Ogata, Masana
Mizugaki, Junko
Izumi, Masaki
Taketa, Kazuhisa
Abstract
Erythrocyte catalase of a Japanese-type acatalasemia case and a normal control subject was separated by chromatofocusing with or without prior partial purification with DEAE cellulose and analyzed by polyacrylamide gradient gel electrophoresis. Two bands of catalase activity were found in the partially purified preparations with molecular weights of 29×10(4) and 35×10(4) in the acatalasemia case and of 28×10(4) and 36×10(4) in the normal control. The molecular weight of normal catalase in untreated hemolysate was 25×10(4). With normal catalase, chromatofocusing fractionation caused a shift toward higher molecular species in the alkaline range eluates from the untreated hemolysate and an opposite shift toward lower molecular weight species in the same pH range eluates from the partially purified preparation. Normal catalase could be identified as protein bands on polyacrylamide gradient gel with hemolysate fractionated by chromatofocusing. Possible application of this technigne in the study of acatalasemia protein with a sensitive protein stain was suggested.
Keywords
無カタラーゼ血症
ポリアクリルアミド・グラジェント・ゲル電気泳動
クロマトフォーカシング
DEAEセルロース・クロマトグラフィー
分子量
Published Date
1982-12-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume94
Issue
issue11-12
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
1003
End Page
1008
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/94/11-12/94_11-12_1003/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma