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ID 16898
Eprint ID
16898
FullText URL
Thumnail 91_685.pdf 2.12 MB
Title Alternative
Studies on specific anti-macrophage serum (AMS) II) Distribution of macrophages in the site of pathology
Author
Miyake, Susumu
Abstract
A cultured human monocytic leukemia cell line, J-III, was used to make heterologous antiserum against human peripheral monocyte surface antigens. To eliminate non-specific antibodies, this antiserum was extensively absorbed with human erythrocytes, neutrophils, nonglass adherent mononuclear cells (mainly lymphocytes) and fetal kidney homogenate. The absorbed serum showed high specificity against human peripheral monocytes, suggesting the presence of similar antigenic determinants on J-III cells and human monocytes. Using this purified serum, an immunofluorescent technique was used to demonstrate macrophages in the site of pathology in various immunological diseases. In synovial tissues from patients with rheumatoid arthritis, positively stained cells were observed in the center and surrounding area of the germinal center. Small numbers of positive cells had also infiltrated around small vessels. Mesangial cells of renal biopsy specimens from patients with SLE were not stained. In Sjogren's disease, salivary glands showed granulomatous change, in which small numbers of diffusely scattered positive cells were observed. Similar changes were seen in Hashimoto thyroiditis. Patients with Behcet's disease showed only a few positive cells in the site of erythema nodosum. There were a small number of infiltrated positive cells in biopsied skin at the site of a positive tuberculin reaction.
Keywords
特異的抗ヒト単球血清の作製
多臓器吸着法
J-111細胞
マクロファージの同定
各種免疫病
Published Date
1979-06-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume91
Issue
issue5-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
685
End Page
695
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/91/5-6/91_5-6_685/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma